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CAREER OBJECTIVE
A research and development position that will allow me
to use my problem-solving skills across a wide range of
analytical chemistry techniques
HIGHLIGHTS
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Method development utilizing a combination of
chromatographic techniques – GC, LC, capillary LC, and
nano LC with atomic spectroscopy/mass spectrometry
detection
·
Extensive knowledge, including maintenance and
troubleshooting, of a broad range of (above mentioned)
analytical methods and instrumentation
·
“Real world” application of current methods for the
analysis of wide variety of analytes in an assortment of
sample matrices
·
Government lab experience
EDUCATION
Ph.D. Analytical Chemistry, University of Cincinnati,
Cincinnati, OH
Advisor: Joseph A. Caruso
Thesis Title “Elemental speciation of phosphorous and
selenium in biological samples”
(GPA – 3.88/4.00), anticipated June 2007.
B.S. Forensic Science (summa cum laude), Eastern
Kentucky University (AAFS accredited program), Richmond,
KY (GPA – 3.71/4.00), 2003.
JOB EXPERIENCE
2003 – present Research Assistant
University of Cincinnati, Cincinnati, Ohio
Advisor: Joseph A. Caruso
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Developed novel approach to explore the various selenium
metabolites in Indian mustard plants, resulting from
genetic modification, using GC or LC with inductively
coupled plasma mass spectrometry (ICP-MS) for detection
§
Determined proxies to quantify the genetic modification
of Indian mustard
§
Identified 3 selenium volatiles previously unreported in
nature
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Developed sample preparation and analysis methods to
evaluate selenium and its association with proteins in
mushrooms
§
Verified the presence of methylselenocysteine and
selenomethionine, possible chemo-preventatives, in
mushrooms
·
Analyzed cerebral spinal fluid for peptide biomarkers
for disease using state-of-the-art Chip-LC-Ion trap-MS
June 2006 – present Joint Research Internship
Food and Drug Administration, Forensic Chemistry Center,
Cincinnati, Ohio
Advisor: Douglas T. Heitkemper
·
Developed novel methods for the separation of chemical
warfare degradation products using LC with ICP-MS and
molecular MS for detection at ultratrace (low ppb)
levels
·
Analyzed U.S. rice for total arsenic levels as well
arsenic speciation using LC-ICP-MS to confirm the safety
of U.S. rice for human consumption.
2003 – present Teaching Assistant
University of Cincinnati, Cincinnati, Ohio
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Supervised laboratory experiments
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Taught students in a classroom setting
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Evaluated student performance
January – June 2003 Summer Internship
Food and Drug Administration, Forensic Chemistry Center,
Cincinnati, Ohio
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Analyzed U.S. potatoes, yams, and wheat samples for
total arsenic levels as well arsenic speciation using
LC-ICP-MS to establish typical levels of arsenic in
previously mentioned foods
AWARDS
AND HONORS
June 2006 Harry B. Mark, Jr., Research Associate
Award – for excellence in graduate research
University of Cincinnati, Cincinnati, OH
January 2006 Poster Presenter Award –
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Winter Conference on Plasma Spectrochemistry, Tucson, AZ
(1 of 2 awarded)
·
NIEHS Superfund Basic Research Program Conference, New
York, NY (1 of 2 awarded)
May 2004 Henry Hochstetter Prize – for
excellence as a graduate teaching assistant
University of Cincinnati, Cincinnati, OH
April 2003 Outstanding Senior Student in
Forensic Science –
Eastern Kentucky University, Richmond, KY
AFFLIATIONS
American Chemical Society
American Academy of Forensic Science
REFERENCES
Dr. Joseph A Caruso Dr. James
Mack Dr. Douglas T.
Heitkemper
Professor Assistant
Professor Inorganic Branch Director
Department of Chemistry Department of
Chemistry Food and Drug Administration
University of Cincinnati University of
Cincinnati Forensic Chemistry Center
joseph.caruso@uc.edu
james.mack@uc.edu
douglas.heitkemper@fda.hhs.gov
(513)556-9306
(513)556-9249 (513)679-2700
ext 187
PUBLICATIONS
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Kubachka, K. M., Richardson, D. R., and Caruso, J. A.,
“Screening for elements and species with LC-ICP-MS.” In
Handbook of Analytical Separations Volume 2: Forensic
Science, 2nd Edition. ed. Bogusz, M.
J.Elsevier, in press.
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Kubachka, K. M., Meija, J., LeDuc, D. L., Terry, N. and
Caruso, J. A. “Selenium volatiles as proxy to the
metabolic pathways of selenium in genetically modified
Brassica juncea.” Environ. Sci. Technol.
2007, DOI: 10.1021/es0613714.
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Munoz, A. H. S., Kubachka, K., Wrobel, K., Corona, F.
G., Yathavakila, S., Caruso, J. A. and Wrobel, K.
"Se-enriched mycelia of Pleurotus ostreatus:
distribution of selenium in cell walls and cell
membranes/cytosol." J. Agric. Food Chem. 2006,
3440-3444.
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Gergely, V., Kubachka, K., Mounicou, M., Fodor, P. and
Caruso, J. A., “Selenium speciation in Agaricus
bisporus and Lentinula edodes mushroom
proteins using multi-dimensional chromatography coupled
to inductively coupled plasma mass spectrometry.”
J. Chromatogr., A.
2006, 94-102.
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Munoz, A. H. S., Kubachka, K., Wrobel, K., Corona, F.
G., Yathavakila, S., Caruso, J. A. and Wrobel, K.
“Metallomics approach to trace element analysis in
Ustilago maydis using cellular fractionation, atomic
absorption spectrometry, and size exclusion
chromatography with ICP-MS detection.” J. Agric. Food
Chem. 2005, 5138-5143.
PRESENTATIONS
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Kubachka, K., Heitkemper, D. H., and Caruso, J. A.
“Detection of chemical warfare agent degradation
products in foods using HPLC-ICP-MS and ESI-MS”; Poster
Presentation at 2007 European Winter Plasma Conference,
19-Feb-2007; Taormina, Italy
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Kubachka, K. "Determining food contamination from
chemical warfare agents using LC-ICP-MS” Invited
Lecture at 2006 Research Associates Seminar
Program. University of Cincinnati. 27-Oct-2006;
Cincinnati, OH
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Kubachka, K., Allen, M. N., Shockey, N. V., and
Heitkemper, D. T., “Arsenic Speciation of Selected US
Rice Samples”; Poster Presentation at 2006 Ralph and
Helen Oesper Banquet and Poster Session, 12-Oct-2005;
Cincinnati, OH
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Kubachka, K. "Elemental Speciation using ICP-MS
(Inductively Coupled Plasma Mass Spectrometry):
Detecting chemical warfare agent degradation products."
Oral Presentation at Eastern Kentucky University,
20-Oct-2006; Richmond, KY
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Kubachka, K., Meija, J., LeDuc, D. L., Terry, N. and
Caruso, J. A. “Monitoring selenium compounds as proxy to
the metabolic pathways of selenium in genetically
modified and wild type Brassica juncea plants”;
Poster Presentation at International Conference on The
Future of Agriculture: Science, Stewardship and
Sustainability, 8-Aug-2006; Sacramento, CA
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Kubachka, K. "Monitoring Selenium Compounds as Proxy to
the Metabolic Pathways
of Selenium in Genetically Modified and Wild Type
Brassica Juncea Plants." Oral Presentation
at 3rd Annual Ohio Mass Spectrometry Symposium,
21-March-2006; Columbus, OH
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Kubachka, K., Meija, J., LeDuc, D. L., Terry, N. and
Caruso, J. A “Monitoring selenium compounds as proxy to
the metabolic pathways of selenium in genetically
modified and wild type Brassica juncea plants”;
Poster Presentation at 2006 Graduate Poster Forum,
3-Mar-2006; Cincinnati, OH.
PRESENTATIONS (continued)
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Kubachka, K., Meija, J., LeDuc, D. L., Terry, N. and
Caruso, J. A “Monitoring selenium compounds as proxy to
the metabolic pathways of selenium in genetically
modified and wild type Brassica juncea plants”;
Poster Presentation at NIEHS Superfund Basic Research
Program Conference, 12-Jan-2006; New York, NY.
·
Kubachka, K., Meija, J., LeDuc, D. L., Terry, N. and
Caruso, J. A “Monitoring selenium compounds as proxy to
the metabolic pathways of selenium in genetically
modified and wild type Brassica juncea plants”;
Poster Presentation at Winter Conference on Plasma
Spectrochemistry, 8-Jan-2006; Tucson, AZ.
·
Kubachka, K., Gergely, V., Montes-Bayon, M and Caruso,
J. A., “Selenium speciation in functional foods using
multi-dimensional chromatography coupled to inductively
coupled plasma mass spectrometry.” Poster Presentation
at BioOhio, 31-Oct-2005; Columbus, OH.
·
Kubachka, K., Meija, J., LeDuc, D. L., Terry, N. and
Caruso, J. A “Monitoring selenium compounds as proxy to
the metabolic pathways of selenium in genetically
modified and wild type Brassica juncea plants”;
Poster Presentation at 2005 Ralph and Helen Oesper
Banquet and Poster Session, 12-Oct-2005; Cincinnati, OH.
·
Kubachka, K., Gergely, V., Fodor, P. and Caruso, J. A.
“Identification of water soluble selenoproteins in
Agaricus bisporus and Lentinula edodes
mushrooms by various chromatographic and mass
spectrometric methods”; Poster Presentation at European
Winter Conference on Plasma Spectrochemistry,
30-Jan-2005; Budapest, Hungary.
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Gergely, V., Kubachka, K., Fodor, P. and Caruso, J. A.
“Speciation of selenium-containing proteins in
selenium-enriched Agaricus bisporus and
Lentinula edodes mushrooms by various
chromatographic techniques coupled to ICPMS”, Poster
Presentation at European Winter Conference on Plasma
Spectrochemistry, 30-Jan-2005; Budapest, Hungary.
·
Kubachka, K., Gergely, V. and Caruso, J. A.
“Characterization of selenium-containing proteins in
selenium-enriched Agaricus bisporus and
Lentinula edodes mushrooms by size exclusion
chromatography and reverse phase ion-pairing
chromatography coupled to ICPMS”; Poster Presentation at
the 2004 Ralph and Helen Oesper Banquet and Poster
Session, 15-Oct-2004; Cincinnati, OH.
·
Kubachka, K., Haplin, P., Heitkemper, D. and Joe Caruso
“Determination of Total and Speciated Arsenic in Samples
of Potatoes, Yams and Wheat Using ICP-MS.”; Poster
Presentation at the 2004 Winter Conference on Plasma
Spectrochemistry, 12-Feb-2004; Fort Lauderdale, FL. |
