Contact: kroninkk@email.uc.edu
Karolin K. Kroening
Dr. Joseph A. Caruso
Karolin is currently pursuing a PhD in Analytical
Chemistry in Dr. Caruso's group at the University of Cincinnati - her
current research is the analysis of degradation products of chemical
warfare agents.
Chemical warfare agents can still represent a problem for the
environment, since their disposal after production during WWI and II is
a high cost procedure and requires special facilities and
transportation. Therefore, these agents are often dumped in the
environment and can undergo undesired reactions producing chemical
warfare degradation products, whose knowledge about toxicity on humans
is limited. Her past two years of research have focused mainly on a
development of a chromatographic separation method using displacement
chromatography for the five main mustard gas hydrolysis degradation
products and their detection with ICPMS. An other interesting project
completed during her second year of research is the cytotoxicity in
monkey kidney cells (CV-1) and speciation study of arsenic containing
chemical warfare degradation products, whose parent agents are
diphenylcyanoarsine (Clark I) and diphenylchloroarsine (Clark II). A
complete study with metallomics approaches has been done on five Clark I
and II degradation products', comparing their toxicity to inorganic (As
III and V) and methylated species (MMA and DMA).While her research has
mostly focused on environmental aspects of metallomics, she has started
transitioning her focus into the biomedical arena. She is currently
studying protein phosphorylation sites by using
capLC-ICPMS and capLC-CHIP/ITMS in cerebral spinal fluid to develop
biomarkers that could trigger interventions to preclude certain stroke
complications.
| Education | |||
| B.S. Chemistry University of Bologna, Italy |
2003 | ||
| M.S. Analytical
Chemistry with major in innovative materials Thesis title: “Synthesis and characterization of polysaccaridic hydroxyapatite compounds” University of Bologna, Italy |
2006 | ||
| Ph.D. student
Analytical Chemistry Division University of Cincinnati, OH |
expected graduation date June 2010 | ||
| Experience | |||
| German and Italian teacher for high schools | 1994-2006 | ||
| Chemist, Innovent
Technologieentwicklung e.V., Jena, Germany Main duties: synthesis of selected ionic polysaccharides, study on morphology and particle size of calcium phosphate particles |
2004 | ||
| Teaching
Assistant General Chemistry Laboratories, University of Cincinnati, OH |
2006-2007 | ||
| Teaching
Assistant Advanced Analytical Laboratory, University of Cincinnati, OH |
2007-present | ||
| Instructor
Advanced Analytical Laboratory, University of Cincinnati, OH |
Summer 2008 | ||
| Instrumental experience | |||
| XRD instrument, Philips Xpert Powder diffractometer | |||
| Transmission Electron Microscope, Philips CM 100 | |||
| Scanning Electron Microscope, Philips XL 30 | |||
| ZETA-SIZER 4 Photon Correlation Spectrometer | |||
| Gas Chromatography Mass Spectrometry | |||
| Fluorescence Spectroscopy | |||
| Atomic Absorption Spectroscopy | |||
| Agilent 7500c/ce/cx Inductively Coupled Plasma-Mass Spectrometer (ICP-MS) | |||
| Agilent 1100 High Performance Liquid Chromatography (HPLC) Apparatus | |||
| Agilent 1200 Capillary High Performance Liquid Chromatography (Cap-HPLC) Apparatus | |||
| Infrared Spectroscopy | |||
| Perkin Elmer Optima 4200 DV Inductively coupled plasma optical emission spectroscopy | |||
| Awards | |||
| Henry Hochstetter
Prize - for excellence in teaching University of Cincinnati, Cincinnati, OH |
May 2008 | ||
|
Ann P. Villalobos Fellowship - for recognition of outstanding research performance by a graduate student; University of Cincinnati, Cincinnati, OH |
June 2009 | ||
| Oral presentations authored and co-authored |
| 1. |
Kirk E. Lokits*, Scott Afton, Karolin Kroening, Renee
Kepler, Patrick A. Limbach, Joseph A. Caruso.
“ICPMS Collision Cell: Novel Applications with Xenon Gas”.
Federation of Analytical Chemistry and Spectroscopy Societies (FACSS), Memphis, TN, October 2007 |
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| 2. | Karolin K.
Kroening, Scott Afton, Kirk Lokits, Doug Richardson, Joseph A.
Caruso. ”Collision/reaction cell ICPMS with applications to
analysis of warfare agent degradation products and
phosphorothioate antisense drugs.” 10th Rio Symposium for Atomic Spectrometry, Salvador, Bahia, Brazil, September 2008 |
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| 3. |
Joseph A. Caruso*, Douglas D. Richardson, Kevin M. Kubachka, Karolin K. Kroening. “Elemental Analysis for Chemical Warfare Agent Degradation Products”. International Atomic Energy Commission, Marine Labs, Monaco, October 2008 |
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| 4. |
Joseph A. Caruso*, Douglas D. Richardson, Kevin M. Kubachka, Karolin K. Kroening. “Elemental Analysis for Chemical Warfare Agent Degradation Products”. University of Pau, CNRS, Pau, France October 2008. |
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| 5. |
Joseph A. Caruso* Douglas D. Richardson, Kevin M. Kubachka, Karolin K. Kroening. “Analyzing Chemical Warfare Agent Degradation Products by ICPMS”. Manchester College, Manchester, IN, December 2008. |
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| 6. |
Karolin K. Kroening, Morwena J. V. Solivio, Scott Afton, Douglas D. Richardson, Joseph A. Caruso. “Screening Different Classes of Chemical Warfare Degradation Products Containing Arsenic and Sulfur by HPLC Coupled with ICPMS and ESI Mass Spectrometry”. Pittsburgh Conference, Chicago, IL, March 2009 |
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| 7. |
Karolin K. Kroening, Douglas D. Richardson, Joseph A. Caruso. “Simultaneous detection of P, S and As chemical warfare agents degradation products with HPLC coupled to ICPMS”. Sixth Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH, April 2009 |
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| 8. |
Karolin K. Kroening, Morwena J. V. Solivio, Scott Afton, Joseph A. Caruso. “Cytotoxicity of arsenic containing chemical warfare agent degradation products with metallomics approaches for metabolite analysis”. International Symposium on Metallomics, Cincinnati, OH, June 2009 |
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| 9. |
Renee N. Easter*, Karolin K. Kroening, Joseph A. Caruso. “Separation and quantification of antisense oligonucleotides by hydrophilic interaction liquid chromatography coupled to ICP-MS”. International Symposium on Metallomics, Cincinnati, OH, June 2009 |
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| 10. |
Karolin K. Kroening, Renee N. Easter, Morwena J. V. Solivio and Joseph A. Caruso. “Cytotoxicity of arsenic containing chemical warfare agent degradation products with metallomics approaches for metabolite analysis”. 57th ASMS Conference on Mass Spectrometry, Philadelphia, PA, June 2009 |
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| 11. |
Renee N. Easter*, Karolin K. Kroening, Joseph A. Caruso. “Separation and quantification of antisense oligonucleotides by hydrophilic interaction liquid chromatography coupled to ICP-MS”. 57th ASMS Conference on Mass Spectrometry, Philadelphia, PA, June 2009 |
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*presenting author
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| Poster presentations |
| 1. |
S. Berger, N. Roveri, B. Palazzo, G. Falini, K. Kroening,
M. Schnabelrauch.
“Hydroxyapatite crystallization in the presence of carboxymethyl
dextran and potential application of the resulting nano-sized
material in bone regeneration.” 19th European Conference on Biomaterials, Sorrento, Italy, September 2005 |
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| 2. | Karolin K. Kroening, Douglas
D. Richardson and Joseph A. Caruso. “Screening for hydrolysis
products of sulfur mustard agents by liquid chromatography with
inductively coupled plasma mass spectrometry.” Central Regional Meeting of the American Chemical Society (CERMACS), Covington, KY, May 2007 |
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| 3. | Karolin K. Kroening, Douglas
D. Richardson and Joseph A. Caruso. “Screening for hydrolysis
products of sulfur mustard agents by liquid chromatography with
inductively coupled plasma mass spectrometry.” Ralph and Helen Oesper Banquet, Poster Session and Symposium, Cincinnati, Ohio, October 2007 |
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| 4. | Karolin K. Kroening, Douglas
D. Richardson and Joseph A. Caruso. “Screening for hydrolysis
products of sulfur mustard agents by liquid chromatography with
inductively coupled plasma mass spectrometry.” 2008 Winter Conference on Plasma Spectrochemistry, Temecula, CA, February 2008 |
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| 5. | Karolin K. Kroening, Scott
Afton, Dean O. Stuart and Jospeh A. Caruso. “Screening
degradation products of organoarsenic-based warfare agents by
liquid chromatography with inductively coupled plasma mass
spectrometry.” 2008 Winter Conference on Plasma Spectrochemistry, Temecula, CA, February 2008 |
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| 6. | Ines R. Coelho,
Karolin K. Kroening, Santha K.V. Yathavakilla, Joseph A. Caruso.
“Determination manganese, nickel and copper concentrations in
tea.” Central Regional Meeting of the American Chemical Society (CERMACS), Columbus, OH, June 2008 |
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| 7. | Coelho I., Kroening K.,
Castanheira I., Caruso J. “HPLC-ICP-MS an emerging technique
for determination of trace elements and elemental species:
applications in food composition data.” 3º EuroFIR Network Meeting, Prague, Czech Republic, September 2008 |
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| 8. | Karolin K. Kroening, Douglas
D. Richardson, Joseph A. Caruso. “Liquid chromatography
coupled with inductively coupled plasma mass spectrometry and
electrospray ionization mass spectrometry studies on hydrolysis
products of sulphur mustard.” Central Regional Meeting of the American Chemical Society (CERMACS), Columbus, OH, June 2008 |
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| 9. | Karolin K. Kroening, Morwena
J.V. Solivio, Mónica García-Lopez, Alvaro Puga and Joseph A.
Caruso. “Metallomics approaches for metabolite analysis of
arsenic containing chemical warfare degradation products.” Ralph and Helen Oesper Banquet, Poster Session and Symposium, Cincinnati, Ohio, October 2008 |
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| 10. | M. García-López, I. Rodríguez,
R. Cela, K. K. Kroening, J. A. Caruso. “Optimization of
organophosphate flame retardants and plasticizers extraction
from sediment samples using MAE and GC-ICP-MS detection.” Ralph and Helen Oesper Banquet, Poster Session and Symposium, Cincinnati, Ohio, October 2008 |
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| 11. | Morwena J.V. Solivio,
Karolin
K. Kroening, Alvaro Puga, Joseph A. Caruso. “Cytotoxicity of
organoarsenic chemical warfare agent degradation products.” Ralph and Helen Oesper Banquet, Poster Session and Symposium, Cincinnati, Ohio, October 2008 |
| Publications |
| 1. |
Karolin K. Kroening, Douglas
D. Richardson, Kevin M. Kubachka, Joseph A. Caruso. Chemical
Warfare Nerve Agents — Analyzing Their Degradation Products. Spectroscopy, 2008; 23(9). |
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| 2. |
Karolin K. Kroening, Morwena
J. V. Solivio, Monica Garcia-Lopez, Alvaro Puga, Joseph A.
Caruso. Cytotoxicity of arsenic-containing chemical warfare
agent degradation products with metallomic approaches for
metabolite analysis. Metallomics, 2009; 1: 59 – 66. |
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| 3. | M. García-López, I. Rodríguez,
R. Cela, K.K. Kroening and J. A. Caruso. Optimization of
organophosphate flame retardants and plasticizers determination
in sediment samples using microwave-assisted extraction and gas
chromatography with inductively coupled. Talanta, 2009; 79(3): 824-829. |
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| 4. |
Karolin K. Kroening, Douglas D. Richardson, Scott Afton, Joseph A. Caruso. Screening hydrolysis products of sulfur mustard agents by HPLC with ICPMS detection. Analytical and bioanalytical chemistry, 2009; 393(8): 1949-56. |
| References |
| Joseph A. Caruso, Professor | Dr. Peter Padolik |
William R. Heineman, Professor |
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| University of Cincinnati | University of Cincinnati |
University of Cincinnati |
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| Department of Chemistry | Department of Chemistry |
Department of Chemistry |
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| Mail Location 0172 | Mail Location 0172 |
Mail Location 0172 |
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| Cincinnati, OH 45221-0172 | Cincinnati, OH 45221-0172 |
Cincinnati, OH 45221-0172 |
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| USA | USA |
USA |
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| Email: Joseph.Caruso@uc.edu | Email: Peter.Padolik@uc.edu |
Email: William.Heineman@uc.edu |
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| Phone: (513)-556-9306 | Phone: (513)-556-9315 |
Phone: (513)-556-9210 |
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| Fax: (513)-556-9239 | Fax: (513)-556-9239 |
Fax: (513)-556-9239 |